University Chapel by the dormitories on the campus of the Ivy League university of Princeton. Founded in 1746, the school is known for its engineering, science and humanities programs. (Getty)
The complaint points to female students who have spoken out against the bathroom policies, including women who told the school newspaper in 2025 that they don’t feel "comfortable" and feel "unsafe."
"In short, the reality of biological sex is a fiction at Princeton University," Defending Education writes.
Additionally, the complaint takes issue with the graduation ceremony practices at Princeton, saying that the prestigious institution offers a ceremony called the "Rose Graduation" which celebrates "women and fem accomplishments and achievements" along with a "Lavender Graduation" that celebrates "queer and trans accomplishments and achievements."
WATCH: STAFF AT TOP UNIVERSITY REVEAL SCHEME TO CONTINUE DEI UNDER 'CHANGED' NAMES
The letter calls for the university to "correct its violations of federal law immediately" and for the Department of Education to "investigate" the "unlawful policies and practices."
Fox News Digital reached out to Princeton University for comment.
"Princeton University, one of the nation’s oldest universities, has a long and prestigious history," Sarah Parshall Perry, vice president and legal fellow at Defending Education, told Fox News Digital.
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Protesters in Michigan rally against President Donald Trump’s anti-DEI policies, denouncing federal rollbacks on diversity, equity, and inclusion programs. (Dominic Gwinn/Getty Images)
"But of late, it has confused sex with gender identity, proudly boasting of its efforts to be one of the most ‘transgender friendly' institutions in the nation. But Princeton’s bathroom, housing, graduation, and medical policies flout federal law, and federal directive, and violate the rights of Princeton’s female students to equal education on the storied campus."
During his first two weeks in office, Trump signed several education-related executive orders on school funding and antisemitism, and launched a federal review of Diversity, Equity, and Inclusion (DEI) practices in federally funded institutions.
Additionally, the Trump administration launched an investigation into a Colorado school district for allegedly "discriminating against its female students" after a girls' restroom was reportedly converted into an "all-gender" facility, while the boys' restroom remained for males only.
During his first week in office, the president launched a federal review of DEI teachings and practices in educational institutions receiving federal funding, in an effort to restore "merit-based opportunity."
Fox News Digital reached out to DOE for comment.
Andrew Mark Miller is a reporter at Fox News. Find him on Twitter @andymarkmiller and email tips to AndrewMark.Miller@Fox.com.
https://www.foxnews.com/politics/top-university-hit-with-scathing-federal-complaint-calling-for-probe-into-unsafe-and-unlawful-dei-agenda